18F-FEC

[18F]FEC ([18F]-Fluoroethylcholine, Fluorocholine (18F) chloride injection) 

 

[18F]-labelled choline analogues, such as 2-[18F]-fluoroethylcholine have been suggested to be useful for the imaging of prostate and brain tumours.  

Indeed, tumour cells with enhanced proliferation rate usually exhibit an improved choline uptake due to the increased membrane phospholipids biosynthesis.  (Asti.M. Nuclear Medicine and Biology, Volume 37, Issue 3, April 2010, Pages 309–315) 

 

The principal tests for [18F]FEC are: 

Test

Technique/instrument

Appearance

Visual

Radionuclidic identity

Gamma-ray spectroscopy with Multi-Channel Analyser (MUCHA)

Radionuclidic identity

Half-life determination with a dose calibrator or alternative detector

pH

pH-meter or pH strips with reading through a reflexion photometer

Impurity kryptofix 222 (aminopolyether)    

Spot test (camera)

Residual solvents including 2- (dimethylamino)ethanol (DMEA) and dibromomethane (DBM)

Gas Chromatography (GC)

Bacterial endotoxines (LAL)

Endosafe Portable Test System (NexGen PTS)

Radiochemical purity

Radio-HPLC (isocratic with UV detetcor)

Radiochemical purity and  [18F]-fluoride

Radio-TLC (miniGITA)

Radiochemical identity

Radio-HPLC

Osmolality (not compulsory)

Osmometer

Radionuclidic purity

Gamma-ray spectroscopy with Multi-Channel Analyser (MUCHA)

Sterility

Compendial method

Radioactivity (volumic activity)

Dose calibrator